Luke Mackinder PI
Luke completed his undergraduate degree in Natural Sciences at Durham University, UK where he graduated in Organic Chemistry and Molecular Biology. During his MSc and PhD, his research focused on viral infection and carbon fixation of marine phytoplankton. Pursuing his passion for understanding carbon fixation in eukaryotic algae, he completed his postdoctoral research in the Jonikas Lab at the Carnegie Institution for Plant Sciences, Stanford USA, where he worked on the carbon concentrating mechanism of the model green algae Chlamydomonas reinhardtii. Since October 2016 he is a lecturer at the University of York where his lab uses systems and synthetic biology approaches to understand eukaryotic and prokaryotic carbon fixation. If he is not in the lab, you'll find Luke riding the roads and trails of Yorkshire on his bike, chasing Yorkshire waves or hanging out with his wife and three daughters.
Abi Perrin Postdoc
I joined the lab and the worlds of microalgae and photosynthesis in January 2021 after a decade working on disease-causing microbes. Before moving to York I completed my degree in Natural Sciences at the University of Cambridge specialising in microbiology then focussed on host-pathogen protein-protein interactions involved in disease during my PhD at the Wellcome Trust Sanger Institute. During this time I became fascinated by the single-celled parasites that hijack red blood cells to cause malaria then moved to London to work more closely on their biology and genetics at the Francis Crick Institute. Now I hope to apply the techniques and insights that have revolutionised our understanding of malaria by using high-throughput genetic approaches to characterise the localisation and function of thousands of proteins in cyanobacteria, which are the photosynthetic prokaryote ancestors of chloroplasts in algae and plants.
Charlotte Walker Postdoc
I completed my BSc in Biological Sciences at the University of Exeter in 2011, after which I left the UK to work on a marine conservation project with the Bahamas National Trust. After 1. 5 years of fine tuning my scientific diving skills and avoiding temperate weather, I returned to the UK to study for a MRes in Marine Biology with Plymouth University. My project focused on the molecular monitoring of harmful algal blooms. During this time I developed a fascination for microalgae physiology and how they influence life on Earth. This led on to my Ph.D research which was conducted with the University of Southampton and the Marine Biological Association of the UK whereby I explored mechanisms of calcification in coccolithophores, photosynthetic marine microalgae which produce calcium carbonate platelets and greatly influence ocean biogeochemistry.
After finishing my Ph.D I have been fortunate to join the Mackinder Lab here in York. I am really interested in physiology and fundamental life processes, therefore the opportunity to work on Chlamydomonas carbon fixation is really exciting. During the project we will be focusing on characterizing HCO3- transport within the carbon concentrating mechanism.
Philipp Girr Postdoc
During my undergraduate education in Bochum and Mainz (Germany), I became highly fascinated by photosynthesis. I joined the lab of Prof. Harald Paulsen (Mainz) in 2015 to study water-soluble chlorophyll proteins, a frequently used model system to understand chlorophyll-chlorophyll as well as chlorophyll-protein interactions.
After I obtained my Ph.D. from the University of Mainz, Germany, I decided to change my research focus from light-harvesting-related research to carbon fixation. After reading more and more about carbon concentrating mechanisms (CCM) in algae, I was very happy to join the Mackinder lab, where I will pursue research on the CCM of Chlamydomonas. My projects in the Mackinder lab will focus on understanding the interaction between thylakoid membrane and pyrenoid, and on building a synthetic CCM model system.
Onyou Nam Postdoc
I completed my undergraduate degree in Biotechnology at Sejong University in Korea. During this time, I became interested in marine phytoplankton. I joined Prof. EonSeon Jin’s lab in 2015 and obtained my PhD in Life Science at Hanyang University in Seoul, Korea. My study focused on the biomineralisation mechanism of the coccolithophorid alga Emiliania huxleyi, which is an important species for the global carbon cycle. While studying microalgae, I got fascinated by carbon concentrating mechanisms (CCM). I was delighted to join the Mackinder lab in May 2021 to understand the fundamental biology of carbon fixation in photosynthetic organisms. My project will focus on developing high-throughput approaches to rapidly advance our knowledge of the diatom CCM. During my free time, I enjoy nature, travelling and like to watch movies and sports.
Irina Grouneva Research technician
Diatom photosynthesis regulation has been the main focus of my research for more than ten years. I obtained my PhD in Biology at the University of Leipzig, Germany, and went on to work as a postdoc in the group of Eva-Mari Aro at the University of Turku, Finland. The main focus of my research during that time was the protein composition, structure and flexibility of the thylakoid membrane of Thalassiosira pseudonana. I moved to Norwich in 2016 to work on Crispr/Cas-mediated targeted gene editing in the ice diatom Fragilariopsis cylindrus in the lab of Thomas Mock at the University of East Anglia.
I joined Luke Mackinder’s lab in February 2018 to support the project identifying and characterising novel proteins involved in CCM and comprising the pyrenoid of Chlamydomonas. I hope to be able to contribute to various aspects of this research topic, providing technical assistance in the fields of cell physiology, proteomics and molecular biology.
Christine Kwok PhD Student
I completed my BSc in Biological Sciences at University of Durham in 2015 and my MSc in Applied Biosciences and Biotechnology at Imperial College London in 2016. I conducted a research project during both of my degrees, with my bachelor one focusing on the genetic diversity of invasive common cordgrass populations from three different locations in England, and my master project looking into the gene expression of two candidate genes that might help control sprouting of potatoes in various temperatures and protein expression and purification of both genes for further structural and interactions study. Throughout my study, I’ve become interested in crop improvement research and hope to contribute to this area in the future. After learning that the Mackinder Lab aims at understanding the CO2-Concentrating Mechanism (CCM) in algae and constructing a functioning one into crop plants to improve photosynthesis for higher crop yields, I immediately applied for the advertised PhD position here. I am grateful that I was accepted and look forward to working here. My project will focus on identifying the regulatory components and pathways in the CCM of Chlamydomonas. If I am not in the lab, I am usually at home listening to music and drawing.
James Barrett PhD Student
I graduated from the University of York with a BSc in Biochemistry. In that time I spent a year at the Technical University of Denmark with Professor Mikael Rørdam Andersen working on Aspergillus fungi. In my final year I completed a BSc research project in the Mackinder lab working on starch-associated proteins in Chlamydomonas. This sparked my interest in green algal research; marrying my interests in microscopy and molecular biology with direct implications for environmental wellbeing. My PhD project in the lab aims to utilise super-resolution microscopy techniques to better understand the dynamics of the phase-separated algal pyrenoid in Chlamydomonas. Outside of the lab, I enjoy road cycling and walking in the Peak District.
Justin Lau PhD Student
I graduated in BSc Plant sciences at the University of Sheffield in 2016. During my degree, I joined Professor Andrew Fleming’s lab group to investigate the impact of stomatal change on A. thaliana histology. At that time I was introduced to the panoply of ways that plant scientists are developing to boost photosynthetic efficiency and improve crop yield. Soon I was fascinated by CCMs, with their often intricate and elegant coupling of physical structure and biochemical pathways. This led to my final year project on light-dark transition of carboxylation/decarboxylation enzyme kinetics in the C4 mechanism in Professor Richard Leegood's group. Continuing this work after graduation, I focused on dissecting enzyme kinetic differences in C4 sub type models. This year, I’m very happy to be in the Mackinder lab, exploring the beauty of another complex CCM. On the weekends, you can normally find me busy in the kitchen making food for my friends for a cosy night in.
Tom Emrich-Mills, current position: PhD student at University of Sheffield